2) Single-cell RNA-sequencing reveals hyperacute NK cell and Monocyte states correlating with poor neurological outcomes after cardiac arrest

Neurological injury remains a significant clinical challenge after successful resuscitation from cardiac arrest (CA). Lymphopenia and elevated cytokine levels suggest a role for the systemic immune response after CA. We determined the immunological network after clinical CA and identified cell states correlating with poor neurological outcomes using single-cell RNA-sequencing. The results suggest the role of native compensatory mechanisms through immune checkpoint axis to maintain homeostasis during acute inflammation and bring immunomodulation as a potential treatment class after CA.

Reference

Tomoyoshi Tamura^#, Changde Cheng^#, Louis T. Merriam, Kohei Ikeda, Kei Hayashida, Mayra Pinilla-Vera, Jack Varon, Sam J. Saliba1, Peter C. Hou, Raghu R. Seethala, Patrick R. Lawler , William M. Oldham, Jiyoung Choi, Immunology of Cardiac Arrest Network (I-CAN), Alexandra J. Weissman, Yohannes Tesfaigzi, Katherine M. Berg, Erin A. Bohula, David A. Morrow, Rebecca M. Baron, Fumito Ichinose, Michael W. Donnino, Xiang Chen, Edy Y. Kim; Single-cell RNA-sequencing reveals hyperacute NK cell and Monocyte states correlating with poor neurological outcomes after cardiac arrest

_{#: Equal contribution.}